🌱 crispio

Command-line utilities and Python API for designing CRISPRi experiments in bacteria.

crispio makes it easy to design annotated and systematically named libraries of guide RNAs. Alternatively, crispio can map a FASTA of existing guides to a genome.

Hint: If you have a table of guide RNAs from the literature that you want to annotate with genomic features, crispio is your tool. Use bioino table2fasta to convert the table to a FASTA file, then use crispio map.

Contents:

• Installation
  • The easy way
  • From source
• Usage
  • Command-line interface
  • Mapping known guide RNAs to a genome
  • Annotating with extra features
  • Checking for off-targets
  • Python API
• crispio
  • crispio package

Issues, problems, suggestions

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Source

View source at GitHub.